![All lanes : Anti-G3BP antibody [EPR13985(B)] (ab181149) at 1/20000 dilutionLane 1 : Hela cell lysate with NFDM/TBSTLane 2 : Jurkat cell lysate with NFDM/TBSTLane 3 : Ramos cell lysate with NFDM/TBSTLane 4 : 293 cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/21683_ab181149-216142-ab181149wb.jpg)
All lanes : Anti-G3BP antibody [EPR13985(B)] (ab181149) at 1/20000 dilutionLane 1 : Hela cell lysate with NFDM/TBSTLane 2 : Jurkat cell lysate with NFDM/TBSTLane 3 : Ramos cell lysate with NFDM/TBSTLane 4 : 293 cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue staining G3BP using ab181149 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.
Immunohistochemical analysis of paraffin-embedded Human muscle tissue staining G3BP using ab181149 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.
Immunofluorescence analysis of 4% paraformaldehyde fixed 293 cells staining G3BP using ab181149 at 1/500 dilution (red), and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution as a secondary antibody. Dapi was used for counterstaining (blue).
Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining G3BP using ab181149 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 dilution as a secondary antibody (red curve). Rabbit monoclonal IgG was the negative control (green curve).
Western blot analysis of G3BP in immunoprecipitation pellets from Ramos cell lysate, using ab181149 at a 1/50 dilution. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST