
ab118512 at 3.75 µg/ml staining GPCR GPR30 in Formalin-Fixed, Paraffin-Embedded Human Adrenal tissue.

ab118512 at 3.75 µg/ml staining GPCR-GPR30 in Formalin-Fixed, Paraffin-Embedded Human Colon tissue.

ab118512 staining G-protein coupled receptor 30 (green) in Baboon placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (7.5µg/ml in PBS + 5% normal horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody. Red - propidium iodide-stained nuclei.See Abreview