ab49960 staining G-CSF in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 7.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris-base buffer. Samples were incubated with primary antibody (1/90 in PBST) for 3 hours at 25°C. A HRP-conjugated Goat anti-rat IgG polyclonal (1/90) was used as the secondary antibody.See Abreview