All lanes : Anti-FMRP [EPR12643] antibody (ab191411) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Anti-FMRP [EPR12643] antibody (ab191411) at 1/50000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling FMRP using ab191411 at 1/100 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab191411.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FMRP using ab191411 at 1/100 dilution (2μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Inset image: negative control obtained using PBS instead of ab191411.
Immunofluorescence analysis of HeLa cells labeling FMRP using ab191411 at 1/200 dilution (4 μg/ml). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.