All lanes : Anti-Flagellin antibody (ab93713) at 1/10000 dilutionLane 1 : 293T transfected with recombinant proteinLane 2 : 293T transfected with recombinant proteinObserved band size : 60 kDa (why is the actual band size different from the predicted?)
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella. Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella. Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.