All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (HRP) (ab196980) at 1/5000 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.