ICC/IF image of ab95940 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95940 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-FGFRL1 antibody (ab95940) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : HepG2 whole cell lysateLane 3 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
ab95940, at a 1/100 dilution, staining FGFRL1 in paraffin-embedded Oral CA Cal27 by Immunohistochemical analysis.