Anti-FGFR4 antibody [5B5]
| Name | Anti-FGFR4 antibody [5B5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab44971 |
| Prices | $398.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 5B5 |
| Applications | IHC-F FC ICC/IF ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Fusion protein corresponding to Human FGFR4 |
| Description | Mouse Monoclonal |
| Gene | FGFR4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab44971 (1/50) detecting fibroblast cells (red). Human BJ fibroblast cells were fixed in formaldehyde, permeabilized in 0.1% Triton and blocked in 8% BSA.
![ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.See Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18893_Fibroblast-Primary-antibodies-ab44971-2.jpg)
ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.See Abreview
ab44971 staining Human primary adult fibroblasts by Flow Cytometry. Cells were harvested with 1 min 0.05% trypsin treatment at 37°C in 5% FBS. Cells were fixed with paraformaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody, (1/50 in 5% FBS), for 20 minutes at 21°C. An Alexa Fluor®647-conjugated Goat polyclonal to mouse IgG (1/400) was used as secondary antibody. See Abreview
![ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/18895_Fibroblast-Primary-antibodies-ab44971-9.jpg)
ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Product References
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Suppressive effect of secretory phospholipase A2 inhibitory peptide on - Suppressive effect of secretory phospholipase A2 inhibitory peptide on
Thwin MM, Douni E, Arjunan P, Kollias G, Kumar PV, Gopalakrishnakone P. Arthritis Res Ther. 2009;11(5):R138.
An immunohistochemical method for identifying fibroblasts in formalin-fixed, - An immunohistochemical method for identifying fibroblasts in formalin-fixed,
Goodpaster T, Legesse-Miller A, Hameed MR, Aisner SC, Randolph-Habecker J, Coller HA. J Histochem Cytochem. 2008 Apr;56(4):347-58. Epub 2007 Dec 10.