Anti-FGFR1 (phospho Y654) antibody
| Name | Anti-FGFR1 (phospho Y654) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59194 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic phosphopeptide (Human) from around the phosphorylation site of tyrosine 654 (DYYPKK) |
| Description | Rabbit Polyclonal |
| Gene | FGFR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of FGFR1 (phospho Y654) staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59194, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-FGFR1 (phospho Y654) antibody (ab59194) at 1/500 dilutionLane 1 : 293 cell extract treated with insulin (0.01U/ml, 15 mins)Lane 2 : 293 cell extract treated with insulin (0.01U/ml, 15 mins) with immunizing phosphopeptide
ICC/IF image of ab59194 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59194, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma - Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma
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