ICC/IF image of ab31739 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31739, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Image courtesy of Human Protein Atlasab31739 staining KDM2A in human skin, showing a distinct nuclear staining pattern in the epidermis. Paraffin-embedded tissue sections (4µm) were incubated with ab31739 (1/75 dilution) for 30 minutes at room temperature. Antigen retrieval by heat induction in citrate buffer pH 6.ab31739 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org