Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.

Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µgSecondaryHRP anti-rabbit IgG (specific to non-reduced IgG) at 1/1000 dilution

Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.

All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (unpurified)Lane 1 : mouse heart lysateLane 2 : rat muscle lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution