Flow cytometric analysis of permeabilized HeLa cells labeling FANCD2 with ab178705 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
All lanes : Anti-FANCD2 antibody [EPR2279(3)] (ab178705) at 1/1000 dilutionLane 1 : HeLa cell lysate with FANCD2 (pS1418) peptideLane 2 : HeLa cell lysate with Non-phospho peptideLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.
All lanes : Anti-FANCD2 antibody [EPR2279(3)] (ab178705) at 1/1000 dilutionLane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate treated with Alkaline PhosphataseLane 3 : HeLa cell lysate treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.