Immunofluorescence analysis using a 1/200 dilution of ab81768. FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope.
Immunfluorescence analysis using a 1/200 dilution of ab81768. FANCD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. Proliferating U2OS cells were exposed to 10 Gy of IR and double color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope.
Anti-FANCD2 antibody (Biotin) (ab81768) at 1/10000 dilution + HeLa whole cell extract