Anti-FANCD2 antibody
| Name | Anti-FANCD2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2187 |
| Prices | $400.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Mouse, Human |
| Antigen | This antibody was made to an N-terminal fusion protein fragment (human) |
| Description | Rabbit Polyclonal |
| Gene | FANCD2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-FANCD2 antibody (ab2187) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
All lanes : Anti-FANCD2 antibody (ab2187) at 1/1500 dilutionLane 1 : Wild type human breast - whole cell lysate (positive control)Lane 2 : Transient D2 Knockdown 24 hours after transfection - whole cell lysateLane 3 : Transient D2 Knockdown 48 hours after transfection - whole cell lysateLane 4 : Transient D2 Knockdown 120 hours after transfection - whole cell lysateSecondaryGoat polyclonal Secondary Antibody to Rabbit IgA - alpha chain (ab2758) at 1/1500 dilution
ab2187 at 1:500 staining FANCD2 in SiHa cells by ICC/IF. The proliferating SiHa cells were exposed to 10 Gy of IR and then double immunofluorescence staining was performed after 8 hours.
ab2187 at 2.5µg/ml staining FANCD2 in human prostate glandular tissue section by Immunohistochemistry (Fromlain/ PFA fixed paraffin-embedded tissue sections).
Product References
A concomitant loss of dormant origins and FANCC exacerbates genome instability by - A concomitant loss of dormant origins and FANCC exacerbates genome instability by
Luebben SW, Kawabata T, Johnson CS, O'Sullivan MG, Shima N. Nucleic Acids Res. 2014 May;42(9):5605-15.
CtIP mediates replication fork recovery in a FANCD2-regulated manner. - CtIP mediates replication fork recovery in a FANCD2-regulated manner.
Yeo JE, Lee EH, Hendrickson EA, Sobeck A. Hum Mol Genet. 2014 Jul 15;23(14):3695-705.
An RNF168 fragment defective for focal accumulation at DNA damage is proficient - An RNF168 fragment defective for focal accumulation at DNA damage is proficient
Munoz MC, Yanez DA, Stark JM. Nucleic Acids Res. 2014 Jul;42(12):7720-33.
Chk1 inhibition as a novel therapeutic strategy for treating triple-negative - Chk1 inhibition as a novel therapeutic strategy for treating triple-negative
Bryant C, Rawlinson R, Massey AJ. BMC Cancer. 2014 Aug 7;14:570.
FANC pathway promotes UV-induced stalled replication forks recovery by acting - FANC pathway promotes UV-induced stalled replication forks recovery by acting
Renaud E, Rosselli F. PLoS One. 2013;8(1):e53693.
ERCC1 and MUS81-EME1 promote sister chromatid separation by processing late - ERCC1 and MUS81-EME1 promote sister chromatid separation by processing late
Naim V, Wilhelm T, Debatisse M, Rosselli F. Nat Cell Biol. 2013 Aug;15(8):1008-15.
Helq acts in parallel to Fancc to suppress replication-associated genome - Helq acts in parallel to Fancc to suppress replication-associated genome
Luebben SW, Kawabata T, Akre MK, Lee WL, Johnson CS, O'Sullivan MG, Shima N. Nucleic Acids Res. 2013 Dec;41(22):10283-97.
RTEL1 contributes to DNA replication and repair and telomere maintenance. - RTEL1 contributes to DNA replication and repair and telomere maintenance.
Uringa EJ, Lisaingo K, Pickett HA, Brind'Amour J, Rohde JH, Zelensky A, Essers J, Lansdorp PM. Mol Biol Cell. 2012 Jul;23(14):2782-92.
The FANC pathway is activated by adenovirus infection and promotes viral - The FANC pathway is activated by adenovirus infection and promotes viral
Cherubini G, Naim V, Caruso P, Burla R, Bogliolo M, Cundari E, Benihoud K, Saggio I, Rosselli F. Nucleic Acids Res. 2011 Jul;39(13):5459-73.
HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination - HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination
Baldeyron C, Soria G, Roche D, Cook AJ, Almouzni G. J Cell Biol. 2011 Apr 4;193(1):81-95.