![All lanes : Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (ab109542) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate treated with Alkaline Phosphatase.Lysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/17451_FANCD2-Primary-antibodies-ab109542-1.JPG)
All lanes : Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (ab109542) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate treated with Alkaline Phosphatase.Lysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
ab109542 (1/200) staining FANCD2 (phospho S1404) in HeLa cells (green). Cells were treated with Bleomycin for 2hrs to induce DNA damage, fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview