All lanes : Anti-FANCB antibody [EPR15513] (ab186729) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-FANCB antibody [EPR15513] (ab186729) at 10000 Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : 293T cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of FANCB in 293T cell lysate immunoprecipitated with ab186729 at 1/50 dilutionSecondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling FANCB with ab186729 at 1/500 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counter stained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling FANCB with ab186729 at 1/120 dilution, compared to a Rabbit monclonal IgG isotype control. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody.