Anti-FANCA antibody
| Name | Anti-FANCA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5063 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: SRSYDHSENSDLVFG-C conjugated to KLH, corresponding to amino acids 995 - 1009 of Human FANCA |
| Description | Rabbit Polyclonal |
| Gene | FANCA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-FANCA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µgSecondaryIRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/17404_ab5063_1.jpg)
Anti-FANCA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µgSecondaryIRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab5063 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5063, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab5063 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5063, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
FANCA knockout in human embryonic stem cells causes a severe growth disadvantage. - FANCA knockout in human embryonic stem cells causes a severe growth disadvantage.
Vanuytsel K, Cai Q, Nair N, Khurana S, Shetty S, Vermeesch JR, Ordovas L, Verfaillie CM. Stem Cell Res. 2014 Sep;13(2):240-50.
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Fanconi anemia core complex gene promoters harbor conserved transcription - Fanconi anemia core complex gene promoters harbor conserved transcription
Meier D, Schindler D. PLoS One. 2011;6(8):e22911.
The Fanconi anemia pathway is downregulated upon macrophage differentiation - The Fanconi anemia pathway is downregulated upon macrophage differentiation
Lu WT, Lemonidis K, Drayton RM, Nouspikel T. Cell Cycle. 2011 Oct 1;10(19):3300-10.
Knockdown of Fanconi anemia genes in human embryonic stem cells reveals early - Knockdown of Fanconi anemia genes in human embryonic stem cells reveals early
Tulpule A, Lensch MW, Miller JD, Austin K, D'Andrea A, Schlaeger TM, Shimamura A, Daley GQ. Blood. 2010 Apr 29;115(17):3453-62.
The E1A-associated p400 protein modulates cell fate decisions by the regulation - The E1A-associated p400 protein modulates cell fate decisions by the regulation
Mattera L, Courilleau C, Legube G, Ueda T, Fukunaga R, Chevillard-Briet M, Canitrot Y, Escaffit F, Trouche D. PLoS Genet. 2010 Jun 10;6(6):e1000983.
FANCG promotes formation of a newly identified protein complex containing BRCA2, - FANCG promotes formation of a newly identified protein complex containing BRCA2,
Wilson JB, Yamamoto K, Marriott AS, Hussain S, Sung P, Hoatlin ME, Mathew CG, Takata M, Thompson LH, Kupfer GM, Jones NJ. Oncogene. 2008 Jun 12;27(26):3641-52.