Lanes 1, 3 & 4 : Anti-eIF4ENIF1 antibody (ab55881) at 1 µg/mlLane 2 : Anti-eIF4ENIF1 antibody (ab55881) at 2 µg/mlLane 1 : Extracts of HeLa cellsLane 2 : Extracts of HeLa cellsLane 3 : Extracts of HeLa cells with eIF4ENIF1 immunizing peptide at 10 µg/mlLane 4 : Extracts of HeLa cells with Non-relevant peptide at 10 µg/mlSecondaryHRP-conjugated Goat anti-Rabbit IgGdeveloped using the ECL technique
IF localization of eIF4ENIF1 in HEK-293T cells stained with ab55881 at 5 mg/ml (A), and DAPI (B). The antibody was detected with a FITC conjugated Goat anti-Rabbit IgG.
All lanes : Anti-eIF4ENIF1 antibody (ab55881) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from HeLa cellsLane 2 : siRNA knockdownLysates/proteins at 10 µg per lane.SecondaryIRDye 800CW conjugated goat anti-rabbit polyclonal at 1/15000 dilution