Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling eIF2B epsilon with ab91565 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-eIF2B epsilon antibody (ab91565) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 5 µgLane 4 : HeLa whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of eIF2B epsilon by Western Blot of Immunprecipitate. ab91565, at 1 µg/ml, staining eIF2B epsilon in HeLa whole cell lysate immunoprecipitated using ab91565 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.