ab87006 staining EFHD2 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in 10mM citrate pH6, 0.1% Tween 20. Samples were incubated with primary antibody (diluted in 0.1% TritonX-100 in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit polyclonal was used as the secondary antibody.See Abreview