
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab126339 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Lane 1 : Lanes 2 - 3 : Anti-Ecat1 antibody (ab126339)Lane 1 : MarkerLane 2 : HEK293T cell lysate - vector only transfectedLane 3 : HEK293T cell lysate - overexpressing Ecat1 with a C-terminal myc-DDK tag (~3.1kDa)

ab126339 at 1/500 dilution staining Ecat1 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.