Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14203(B)]

Name	Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14203(B)]
Supplier	Abcam
Catalog	ab180125
Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR14203(B)
Applications	WB FC IHC-P ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human E1 Ubiquitin Activating Enzyme aa 200-300
Description	Rabbit Monoclonal
Gene	UBA1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.

Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

All lanes : Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14203(B)] (ab180125) at 1/5000 dilutionLane 1 : Fetal brain lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution