Anti-Dystrobrevin alpha [EPR14112] antibody

Name	Anti-Dystrobrevin alpha [EPR14112] antibody
Supplier	Abcam
Catalog	ab191395
Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR14112
Applications	WB IHC-P ICC/IF ICC/IF FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Dystrobrevin alpha aa 650 to the C-terminus
Description	Rabbit Monoclonal
Gene	DTNA
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Dystrobrevin alpha [EPR14112] antibody (ab191395) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human cerebellum lysateLane 3 : Human skeletal muscle lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of U87-MG cells (4% formaldehyde-fixed; 0.1% Triton X-100 permeabilized) labeling Dystrobrevin alpha with ab191395 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at 1/200 dilution. Counter-stained with DAPI (blue). Negative controls: Primary ab concentration (anti-DTNA) is 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

All lanes : Anti-Dystrobrevin alpha [EPR14112] antibody (ab191395) at 1/10000 dilutionLane 1 : A673 cell lysateLane 2 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Dystrobrevin alpha with ab191395 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

Flow Cytometric analysis of A673 cells (paraformaldehyde-fixed, 2%) labeling Dystrobrevin alpha with ab191395 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.