Anti-Dynein intermediate chain 1 antibody [EPR11244-61]

Name	Anti-Dynein intermediate chain 1 antibody [EPR11244-61]
Supplier	Abcam
Catalog	ab171964
Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR11244-61
Applications	WB ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Dynein intermediate chain 1
Description	Rabbit Monoclonal
Gene	DNAI1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.

All lanes : Anti-Dynein intermediate chain 1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)Lane 1 : Human testis lysateLane 2 : rat testis lysateLane 3 : mouse testis lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Immunofluorescence analysis of Jurkat cells, labeling Dynein intermediate chain 1 using unpurified ab171964 at a 1/50 dilution (green) and DAPI nuclear staining (blue).